Cancer functional genomics lab
-
Liu L, Neve N, Perlaza-Jimenez L, Xi X, Purcell J, Hawdon A, Conn SJ, Zenker J, Tamayo P, Goodall GJ, Rosenbluh J. Systematic loss of function screens identify pathway specific functional circular RNAs. Nature Cell Biology, 26(8)1359-1372, 2024.
-
Kulkarni A, Mohan V, Tang TT, Post L, Chan YC, Manning M, Thio N, Parker BL, Dawson MA, Rosenbluh J, Vissers JH, Harvey KF. Identification of resistance mechanisms to small-molecule inhibition of TEAD-regulated transcription. EMBO Reports, 25:3944-3969, 2024.
-
Wang L, Bitar M, Lu X, Jacquelin S, Nair S, Sivakumaran H, Hillman KM, Kaufmann S, Ziegman R, Casciello F, Gowda H, Rosenbluh J, Edwards SL, French JD. CRISPR-Cas13d screens identify KILR, a breast cancer risk-associated lncRNA that regulates DNA replication and repair. Molecular Cancer, 23(1):101, 2024.
-
Voon HP, Hii L, Garvie A, Udugama M, Krug B, Russo C, Chüeh AC, Daly RJ, Morey A, Bell TD, Turner SJ, Rosenbluh J, Daniel P, Firestein R, Mann JR, Collas P, Jabado N, Wong LH. Pediatric glioma histone H3. 3 K27M/G34R mutations drive abnormalities in PML nuclear bodies. Genome Biology, 23(1):284, 2023.
-
Ghamsari R, Rosenbluh J, Menon V, Lovell NH, Alinejad-Rokny H. Technological Convergence: Highlighting the Power of CRISPR Single-Cell Perturbation Toolkit for Functional Interrogation of Enhancers. Cancers, 15(4): 3566, 2023.
-
Tuano NK, Beesley J, Manning M, Shi W, Perlaza-Jimenez L, Malaver-Ortega LF, Paynter J, Black D, Civitarese A, McCue K, Hatzipantelis A, Hillman K, Kaufmann S, Sivakumaran H, Polo JM, Reddel R, Band V, French JD, Edwards SL, Powell D, Chenevix-Trench G, Rosenbluh J. CRISPR screens identify gene targets at breast cancer risk loci. Genome Biology, 2023, 24(1):59.
-
Sun CX, Daniel P, Bradshaw G, Shi H, Loi M, Chew N, Parackal S, Tsui V, Liang Y, Koptyra M, Adjumain S, Sun C, Chong WC, Fernando D, Drinkwater C, Tourchi M, Habarakada D, Sooraj D, Carvalho D, Storm PB, Baubet V, Sayles LC, Fernandez E, Nguyen T, Porksen M, Doan A, Crombie D, Panday M, Zhukova N, Dun MD, Ludlow LE, Day B, Stringer B, Neeman N, Rubens J, Raabe EH, Vinci M, Tyrrell V, Fletcher JI, Ekert PG, Dumevska B, Ziegler DS, Tsoli M, Sulaiman SNF, Loh AHP, Low SY, Sweet-Cordero A, Monje M, Resnick A, Jones C, Downie P, Williams B, Rosenbluh J, Gough D, Cain JE, Ron Firestein R. Generation and Multi-Dimensional Profiling of a Childhood Cancer Cell Line Atlas Defines New Therapeutic Opportunities. Cancer Cell, 2023, S1535-6108(23)00080-6.
-
Dowty JG, Yu C, Hosseinpour M, Joo JE, Wong EM, Nguyen-Dumont T, Rosenbluh J, Giles GG, Milne RL, MacInnis RJ, Dugué PA, Southey MC. Heritable methylation marks associated with prostate cancer risk. Familial Cancer, 2023
-
Davies R*, Liu L*, Taotao S, Tuano N, Chaturvedi R, Huang K, Itman C, Amit M, Qamra A, Hu C, Powell D, Daly R, Tan P, Rosenbluh J. CRISPRi enables isoform specific loss-of-function screens and identification of gastric cancer specific isoform dependencies. Genome Biology, 2021, 22(1):47.
-
Curvello R, Kerr G, Micati D, Chan W, Raghuwanshi V, Rosenbluh J, Abud H, Garnier G. Engineered Plant-Based Nanocellulose Hydrogel for Small Intestinal Organoid Growth. Advance Sciences, 2020, 8(1):2002135.
-
Beesley J, Sivakumaran H, Moradi Marjaneh M, Lima LG, Hillman KM, Kaufmann S, Tuano N, Hussein N, Ham S, Mukhopadhyay P, Kazakoff S, Lee JS, Michailidou K, Barnes DR, Antoniou AC, Fachal L, Dunning AM, Easton DF, Waddell N, Rosenbluh J, Möller A, Chenevix-Trench G, French JD, Edwards SL. Chromatin interactome mapping at 139 independent breast cancer risk signals. Genome Biology, 2020 Jan 7;21(1):8.
-
Li T, Kim A, Rosenbluh J, Horn H, Greenfeld L, An D, Zimmer A, Liberzon A, Bistline J, Natoli T, Li Y, Tsherniak, Narayan R, Subramanian A, Liefeld T, Wong B, Thompson D, Calvo S, Carr S, Boehm J, Jaffe J, Mesirov J, Hacohen N, Regev A, Lage K. GeNets: a unified web platform for network-based genomic analyses. Nature Methods, 2018 15,543-546.
-
Rosenbluh J, Xu , Harrington W, Gill S, Wang X, Vazquez F, Root DE, Tsherniak A, Hahn WC. Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression. Nature Communications, 2017, 8:15403.
-
Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, Piccioni F, Horn H, Fagbami L, Yang-Zho D, Perrimon N, Jaffe J, Lage K, Boehm JS, Hahn WC. Integrated genetic and proteomic interrogation of WNT/β-catenin cancer dependencies. Cell Systems, 2016, 3(3):302-316.
-
Kim JW, Botvinnik OB, Abudayyeh O, Birger C, Rosenbluh J, Shrestha Y, Abazeed ME, Hammerman PS, DiCara D, Konieczkowski DJ, Johannessen CM, Liberzon A, Alizad-Rahvar AR, Alexe G, Aguirre A, Ghandi M, Greulich H, Vazquez F, Weir BA, Van Allen EM, Tsherniak A, Shao DD, Zack TI, Noble M, Getz G, Beroukhim R, Garraway LA, Ardakani M, Romualdi C, Sales G, Barbie DA, Boehm JS, Hahn WC, Mesirov JP, Tamayo P. Nature Biotechnology, 2016, 34(5):539-546.
-
Shao DD, Xue W, Krall, EB, Bhutkar A, Piccioni F, Wang X, Schinzel AC, Sood S, Rosenbluh J, Kim WJ, Zwang Y, Root DE, Jacks T, Hahn WC. KRAS and YAP1 converge to regulate EMT and tumor survival. Cell, 2014 158(1):171-184.
-
Giannakis M, Hodis E, Mu J, Yamauchi M, Rosenbluh J, Cibulskis K, Saksena G, Lawrence MS, Van Allen EM, Hahn WC, Gabriel SB, Lander ES, Getz G, Ogino S, Fuchs CS, Garraway LA. RNF43 is frequently mutated in colorectal and endometrial cancers. Nature Genetics, 2014 46:1264-66.
-
Rosenbluh J, Wang X, Hahn WC. Genomic insights into WNT/β-catenin signaling. Trends in Pharmacological Science, 2014 Feb;35(2):103-9.
-
Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P, Mesirov JP, Beroukhim R, Hahn WC. Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer drivers. Cancer Discovery, 2013 3(9):1044-57.
-
Rosenbluh J, Nijhawan D, Cox AG, Li X, Neal JT, Schafer EJ, Zack TI, Wang X, Tsherniak T, Schinzel AC, Shao DD, Schumacher SE, Weir BA, Vazquez F, Cowley GS, Root DE, Mesirov JP, Beroukhim R, Kuo CJ, Goessling W, Hahn WC. β-catenin driven cancers require a YAP1 transcriptional complex for survival and tumorigenesis. Cell, 2012 151(7):1457-1473.
-
Nijhawan D, Zack TI, Ren Y, Strickland MR, Lamothe R, Schumacher SE, Tsherniak A, Besche HC, Rosenbluh J, Shehata S, Cowley GS, Weir BA, Goldberg AL, Mesirov JP, Root DE, Bhatia SN, Beroukhim R, Hahn WC. Cancer vulnerabilities unveiled by genomic loss. Cell, 2012 150(4): 842-54.
-
Lovejoy CA, Li W, Reisenweber S, Thongthip S, Bruno J, de Lange T, De S, Petrini JH, Sung PA, Jasin M, Rosenbluh J, Zwang Y, Weir BA, Hatton C, Ivanova E, Macconaill L, Hanna M, Hahn WC, Lue NF, Reddel RR, Jiao Y, Kinzler K, Vogelstein B, Papadopoulos N, Meeker AK. Loss of ATRX, genome instability, and an altered DNA damage response are hallmarks of the alternative lengthening of telomeres pathway. PLoS Genetics, 2012;8(7):e1002772.
* All authors have contributed equally and are listed by lab.
-
Rosenbluh J, Nijhawan D, Chen Z, Wong KK, Masutomi K, Hahn WC. RMRP Is a Non-Coding RNA Essential for Early Murine Development. PLoS One, 2011;6(10):e26270.
-
Wiedemeyer WR, Dunn IF, Quayle SN, Zhang J, Chheda MG, Dunn GP, Zhuang L, Rosenbluh J, Chen S, Xiao Y, Shapiro GI, Hahn WC, Chin L. Pattern of retinoblastoma pathway inactivation dictates response to CDK4/6 inhibition in GBM. PNAS, 2010;107(25):11501-6.
-
Bugatti A, Chiodelli P, Rosenbluh J, Loyter A, Rusnati M. BSA conjugates bearing multiple copies of the basic domain of HIV-1 Tat: Prototype for the development of multitarget inhibitors of extracellular Tat. Antiviral-Research, 2010;87(1):30-9.
-
Levin A, Armon-Omer A, Rosenbluh J, Melamed-Book N, Graessmann A, Waigmann E, Loyter A. Inhibition of HIV-1 integrase nuclear import and replication by a peptide bearing integrase putative nuclear localization signal. Retrovirology, 2009;6:112.
-
Levin A, Rosenbluh J, Hayouka Z, Friedler A, Loyter A. Integration of HIV-1 DNA is regulated by interplay between viral rev and cellular LEDGF/p75 proteins. Molecular-Medicine, 2010;16(1-2):34-44.
-
Hayouka Z, Rosenbluh J, Levin A, Loyter A, Friedler A. Peptides derived from HIV-1 Rev inhibit HIV-1 Integrase in a shiftide mechanism. Biopolymers, 2008;90:481-487.
-
Rosenbluh J, Hayuka Z, Loya S, Levin A, Armon-Omer A, Britan E, Hizi A, Kotler M, Friedler A, Loyter A. Interaction between HIV-1 Rev and integrase proteins: A basis for the development of Anti-HIV peptides. Journal of Biological Chemistry, 2007;282:15743-15753.
-
Hayouka Z, Rosenbluh J, Levin A, Loya S, Lebendiker M, Veprintsev D, Kotler M, Hizi A, Loyter A, Friedler A. Shiftides: Peptides that Inhibit HIV-1 Replication by Shifting the Oligomerization Equilibrium of the Viral Integrase. PNAS, 2007;104: 8316-8321.
-
Kass G, Arad G, Rosenbluh J, Gafni Y, Graessmann A, Rojas MR, Gilbertson RL, Loyter A. Permeabilized mammalian cells as an experimental system for nuclear import of geminiviral karyophilic proteins and of synthetic peptides derived from their nuclear localization signal regions. Journal of General Virology, 2006;87:2709-2720.
-
Rosenbluh J, Kapelnikov A, Shalev DE, Rusnati M, Bugatti A, Loyter A. Positively charged peptides bearing nuclear localization signals are able to interact with each other as revealed by solid phase binding assay systems. Analytical-Biochemistry, 2006;352:157-168.
-
Hariton-Gazal E, Rosenbluh J, Zakai N, Fridkin G, Brack-Werner R, Wolff H, Devaux C, Gilon C, Loyter A. Functional Analysis of Backbone Cyclic Peptides Bearing the Arm Domain of the HIV-1 Rev Protein: Characterization of the Karyophilic Properties and Inhibition of Rev-Induced Gene Expression. Biochemistry, 2005;44:11555-11566.
-
Loyter A, Rosenbluh J, Zakai Z, Li J, Kozlovsky SV, Tzfira T, Citovsky V. The Plant VirE2 Interacting Protein 1 A Molecular Link between the Agrobacterium T-Complex and the Host Cell Chromatin. Plant-Physiology, 2005;138:1318-1321.
-
Rosenbluh J, Hariton-Gazal E, Dagan A, Rottem S, Graessmann A, Loyter A. Translocation of histone proteins across lipid bilayers and mycoplasma membranes. Journal of Molecular Biology, 2005;345,387-400.
-
Rosenbluh J, Kumar Singh S, Gafni Y, Graessmann A, Loyter A. Non-endocytic penetration of core histones into petunia protoplasts and cultured cells: A novel mechanism for the introduction of macromolecules into plant cells. Biochimica Biophysica Acta , 2004;1664:230-240.
-
Hariton-Gazal E, Rosenbluh J, Graessmann A, Gilon C, Loyter A. Direct translocation of histone molecules across cell membranes. Journal of Cell Science, 2003;116:4577-4586.